Säsongs- och extrajobb
Context Immunofluorescence (IF) is a powerful technology that enables visualizing proteins inside of cells using fluorescently labeled antibodies. Combined with high resolution confocal microscopy the localization of proteins can be annotated to different cellular organelles and even sub-organelle structures like small bodies in the nucleus. This technology is systematically used in the Human Protein Atlas (HPA) to map the human proteome at a subcellular level. To achieve this a panel of different cell lines are used and based on transcriptomics data the cell lines most suitable for detection of each protein is selected for IF. Most of the cell lines are adherent cells, which means they attach to surfaces and thus it is relatively easy to distinguish between different cellular structures and today we have subcellular data for ≈ 60 % of the human proteome. However, many proteins are not expressed in adherent cells but primarily in hematopoietic cells (cells of the blood and the immune system). These cells grow in suspension and do not attach to surfaces. They are relatively small with a large nucleus and a small cytosol. This makes it harder to prepare the cells for imaging and to resolve the different cellular structures. Yet, they are highly valuable in the work of mapping the human proteome because of the expression of many proteins that cannot be detected in the adherent cells. We have established a protocol for preparing the cells for IF but more work is needed to better distinguish between the cellular structures in these cells. Your Project You will work with different types of suspension cells used in the HPA with the aim to establish a better protocol for IF of these cells. One approach for doing this is to use a new technology called expansion microscopy that literally magnifies the cells and by this better resolution of cell structures can be obtained. The project will include mammalian cell cultivation, wet lab work (in principle immunostaining and optimization of different parameters in this protocol), imaging with confocal and wide field microscopes, but mostly wet-lab work relating to the development of the expansion protocol. Work environment You will be hosted by the division of cellular and clinical proteomics at the School of Engineering Sciences in Chemistry, Biotechnology and Health. The research group is called Cell profiling and is headed by Dr Emma Lundberg who is also the Director of the HPA Cell Atlas. We are located at Scilifelab in Solna and are 15 people working in the group. Contact Contact person: Charlotte Stadler Email: firstname.lastname@example.org For more info of the HPA and the research group please visit www.proteinatlas.org and https://www.scilifelab.se/researchers/emma-lundberg/
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Requirements for MSc students. We are looking for enthusiastic, motivated students, who enjoy working as part of a team as well as independently. Ideally, candidates have some previous practical experience in methods used in molecular biology, cell cultivation or microscopy. Please send us a short description of your relevant work experience, your CV, and your motivation.
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